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Notes on daily use of liquid nitrogen biological container

Time: 2020-09-22 10:43 Source: unknown Author: Bander liquid nitrogen tank Click: second
In order to make the breeder use it more safely and normally in the freezing process liquid nitrogen tank Here are some points for attention.

1. Storage of liquid nitrogen biological container

1.1 liquid nitrogen is a kind of ultra-low temperature substance, and the temperature can reach - 196 ℃. When operating, you should pay attention to your own protection to avoid frostbite. When using liquid nitrogen indoors, pay attention to ventilation. It is measured that a volume of liquid nitrogen can evaporate to 700 times the volume of gaseous nitrogen, resulting in the reduction of oxygen content in the same volume of air. The oxygen content in normal air is 21%. If the oxygen content is lower than 16%, it will affect human health.

1.2 it is strictly forbidden to place articles on the cover plug of the liquid nitrogen biological container, so that the vaporized nitrogen can overflow naturally. It is forbidden to use self-made cap plug to close the tank mouth, so as to avoid the tank body damage caused by the increase of internal pressure of liquid nitrogen biological container, which may cause safety problems seriously.

1.3 in order to effectively reduce the loss of liquid nitrogen, the biological container of liquid nitrogen should be placed on a firm support which is not exposed to sunlight, far away from heat source and about 25cm above the ground. The bracket can be made of angle iron welded frame, covered with wood and other materials with poor thermal conductivity.

one point four liquid nitrogen tank In the use process, pay attention to observation. Under normal conditions, the temperature of the tank body is the same. After removing the cover plug, the fog in the tank mouth will not overflow and sink. If it is found that there are water drops or frosting on the lid plug and the upper part of the tank, or if you feel the upper part is cold and the lower part is hot when you touch the outer wall of the tank with your hand, and listen to the sound of bubbles in the jar frequently with your ears at the mouth of the tank, it indicates that the quality of the liquid nitrogen biological container has been in question, and the liquid nitrogen storage time is shorter, so it should be used cautiously or stopped.

1.5 in order to insulate the tank body, the tank wall is designed as a double-layer vacuum structure. However, this structure makes the outer wall of the tank bear a lot of atmospheric pressure. If it is impacted by strong turbulence, vibration and other external forces, it is easy to cause damage to the liquid nitrogen biological container. Therefore, special attention should be paid to the fixation of the liquid nitrogen biological container during transportation. In practical application, if it is used for transportation, liquid nitrogen storage tank should be selected. This type of tank is not only suitable for indoor standing, but also suitable for long-distance transportation.

2. Use and maintenance of liquid nitrogen biological container

two point one liquid nitrogen tank In the process of use, it is necessary to check the amount of liquid nitrogen in the tank so as to supplement it in time. Taking the national standard 10L tank as an example, if the distance between the liquid level and the upper opening of the container is more than 11cm, or the concentration from the liquid level to the bottom of the tank is less than 18cm, liquid nitrogen should be added once every 20 days. In practice, if it is convenient to measure the weight, the amount of liquid nitrogen should not be less than 11.6kg. If it can not be replenished in time under special circumstances, the stored materials can be taken out from the lifting cylinder and directly sunk into the bottom of the tank, and then the liquid nitrogen can be replenished as soon as possible.

2. Pay attention to the use of liquid nitrogen storage container. Frozen semen must be stored in liquid nitrogen. When the temperature rises from - 196 ℃ to - 100 ℃ - 70 ℃, sperm will be damaged. When the frozen semen is transferred to another liquid nitrogen biological container, the frozen semen should not be separated from liquid nitrogen for more than 5 seconds. It is forbidden to take the liquid nitrogen out of the container for more than 10s. If the semen is not taken out within 10 seconds, the lifting cylinder or gauze bag should be applied to liquid nitrogen immediately, and it can be taken out again after 10-15 seconds.

2.3 most of the liquid nitrogen currently used is produced in batch in the factory, with many impurities. In addition, the impurities brought in during the storage and retrieval of frozen semen are easy to pollute the tank. Generally, the liquid nitrogen biological container should be cleaned once a year. It can be washed with neutral detergent first and then washed with warm water at about 35 ℃. After cleaning, the tank should be fully dried and then filled with liquid nitrogen for use.

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